Consulta: subjectFacets:"Cryopreservation"
Registros recuperados: 69
Data/hora: 19/05/2024 10:37:19

A comparative study in direct cryopreservative efficacy between Triladly® and EDTA saline glucose 10% glycerol cryopreservative media for human and non-human infective trypanosomes
Provedor de dados: 19
Autores: Ndungu, K; Gitonga, P; Mulinge, M; Kangethe, J; Kibugu, J. K; Munga, L. K; Maina, N; Kagira, J. M; Ngae, G. N; Murilla, G.
The efficacy of Triladyl®, a commercial cryomedium for bull semen, in the cryopreservation of both human and animal infective trypanosomes as compared to EDTA Saline Glucose (ESG) 10% glycerol was evaluated in the current study. Cryopreserved Trypanosoma brucei rhodesiense, T. evansi, T. b. brucei and T. congolense were first propagated in irradiated mice. At the peak of parasitemia, parasites were harvested by cardiac puncture and 106,105,104103,102 and 10 dilutions made using whole blood bled from clean mice. These dilutions were divided into two equal portions of 0.5 ml each and cryopreserved in both ESG 10% glycerol and neat Triladly®. The procedure was also repeated with T. congolense and T. vivax species of trypanosomes directly isolated from...
Palavras-chave: Trypanosoma; Cryopreservation.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2500

Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
Provedor de dados: 48
Autores: de los Reyes,Mónica; Palomino,Jaime; Martínez,Víctor; Aretio,Carolina; Gutiérrez,Michel.
We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. After incubation, the samples were assessed by the indirect immunofluorescent staining technique. The probability of having unlabeled sperm (PUS), indicating acrosin loss, was modelled by a binomial distribution using logistic regression. There was a linear relationship between PUS and time at both temperatures (p&lt;0.001); however, a major percentage of unlabeled...
Tipo: Journal article
Palavras-chave: Acrosin; Capacitation; Dog sperm; Cryopreservation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200005

Advantages and Applications of Cryopreservation in Fisheries Science
Provedor de dados: 52
Autores: Tsai,S.; Lin,C.
Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014

Artificial Insemination with Canine Spermatozoa Frozen in a Skim Milk/Glucose-Based Extender
Provedor de dados: 19
Autores: ABE, Yasuyuki; LEE, Dong-Soo; SANO, Hikaru; AKIYAMA, Koji; YANAGIMOTO-UETA, Yoshiko; ASANO, Tomoyoshi; SUWA, Yoshinori; SUZUKI, Hiroshi; 鈴木, 宏志.
Due to the recent outbreak of avian influenza, transportation of frozen canine semen with egg yolk has been sharply restricted. Thus, there is urgent need to develop a novel egg yolk-free extender for freezing canine spermatozoa. In the present study, the effect of using skim milk/glucose (SG)-based extender without egg yolk on the motility and fertilizing capacity of canine spermatozoa frozen-thawed in the presence of glycerol was examined. There was a tendency for the proportion of motile spermatozoa exposed to SG-based extender for 3 h to be higher than that exposed for 1 h, but the difference was not significant. The motility and other viability parameters of canine spermatozoa after thawing were similar to those obtained with an egg yolk-based...
Palavras-chave: Canine; Cryopreservation; Skim milk/glucose-based extender; Spermatozoa.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3030

Assessment of the interaction between straw size and thawing rate and its impact on in vitro quality of post-thaw goat semen
Provedor de dados: 96
Autores: Bezerra,Francisco Silvestre Brilhante; Castelo,Thibério de Souza; Santos,Érika Aparecida Araújo dos; Dantas,Tiago da Costa; Simão,Bruno Rodrigo; Silva,Alexandre Rodrigues.
The objective of this study was to analyze interactions between different straw sizes and thawing rates on the post-thaw goat semen parameters. Twenty-one ejaculates (seven per animal) were collected from three stud bucks by using an artificial vagina. After evaluation, the semen was extended in Tris-egg yolk-glycerol and packed in 0.25 and 0.50 mL straws, followed by storage in liquid nitrogen. Thawing was performed using two different rates: 37 ºC/1 min and 55 ºC/7 s. The interaction between the 0.5-mL straw and the thawing rate of 55 ºC/7 s promoted higher progressive motility. When the effect of straws alone was analyzed, it was verified that the use of the 0.50 mL straw promoted better conservation than the 0.25 mL one for progressive motility and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capra hircus; Cryopreservation; Semen processing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000300016

Banco de sementes de café em criopreservação: experiência inédita no Brasil.
Provedor de dados: 14
Autores: EIRA, M. T. S.; REIS, R. B.; RIBEIRO, F. N. S.; RIBEIRO, V. S.
bitstream/CENARGEN/26622/1/ct042.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Banco; Coffee; Bank.; Café; Armazenamento; Criopreservação; Germinação; Germoplasma.; Coffea; Germination; Germplasm.; Cryopreservation.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/187030

(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?
Provedor de dados: 45
Autores: Silva,E.C.B.; Arruda,L.C.P.; Vieira,J.I.T.; Soares,P.C.; Guerra,M.M.P.
ABSTRACT The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P&lt;0.05) with 15µM than with 50 and 100; WOB was lower (P&lt;0.05) with 100µM than with 0, 15, and 25; and BCF was higher (P&lt;0.05) with 75 and 100µM than with 0. In turn, in Experiment 2, progressive motility was higher (P&lt;0.05) with0 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Cryopreservation; Flavonoids; Oxidative stress; Semen.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200521

Continuous in vitro Cultivation of Babesia ovata
Provedor de dados: 19
Autores: Igarashi, Ikuo; Avarzed, Avgaandorjiin; Tanaka, Tetsuya; Inoue, Noboru; Ito, Masanari; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi.
Palavras-chave: Babesia ovata; In vitro culture; Low oxygen atmosphere; Cryopreservation.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/212

Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
Provedor de dados: 96
Autores: Machado,Gilmara Junqueira; Murgas,Luis David Solis; Palhares,Priscila Cotta; Assis,Isadora de Lima; Castro,Tássia Flávia Dias.
ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Biotechnology; Cryopreservation; Eggs; Fish; Larvae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155

Cooling of ejaculated and epididymal stallion sperm
Provedor de dados: 45
Autores: Monteiro,G.A.; Guasti,P.N.; Hartwig,F.P.; Dellaqua Jr.,J.A.; Alvarenga,M.A.; Papa,F.O.
After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recovered from the epididymal cauda after storage for 24 hours at 5°C (G3). To obtain G1 sperm, two ejaculates were collected. After 1 week, all stallions underwent a bilateral orchiectomy, and one of the removed epididymides was flushed to obtain G2 sperm. The contralateral epididymis was stored at 5°C for 24 hours before being flushed to obtain G3 sperm. The sperm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse epididymal spermatozoa; Cryopreservation; Stallion; Sperm viability; Seminal plasma.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000300010

Cryopreservation of an avian spirochete strain in liquid nitrogen
Provedor de dados: 45
Autores: Labruna,M.B.; Resende,J.S.; Martins,N.R.S.; Jorge,M.A.
Soros de aves experimentalmente infectadas, contendo espiroquetas viáveis, foram submetidos a dois procedimentos antes da criopreservação: glicerol na diluição de 1/2 (v/v), designado como soro com glicerol a 50% (GS), e dimetilsulfóxido na proporção de 1/10 (v/v), designado como soro com DMSO a 10% (DS). Após 15 meses de estocagem em nitrogênio líquido, amostras dos tratamentos GS e DS foram descongeladas e suas infectividades foram testadas em frangos susceptíveis. Apesar de ambos os procedimentos terem mantidos a infectividade da bactéria, DMSO a 10% no soro de frango apresentou-se mais satisfatório como criopreservante.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chicken; Spirochete; Borrelia anserina; Cryopreservation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000600008

Cryopreservation of Crassostrea gigas vesicular cells: Viability and metabolic activity
Provedor de dados: 5
Autores: Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m.
Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into...
Tipo: Text
Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf

Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly
Provedor de dados: 62
Autores: VISINTIN,José Antônio; GARCIA,José Fernando; PANTANO,Thais; D’ÁVILA ASSUMPÇÃO,Mayra Elena Ortiz.
Compacted mouse morulae were frozen at 0.3ºC/min. or 0.5ºC/min. from -6ºC to -24ºC or -32ºC in 10% of glycerol plus different sucrose concentrations with or without 0.1% of honeybee royal jelly. Embryos were thawed in water bath at 22ºC for 20 seconds and cryoprotectant dilution was done in three steps. Embryos were cultured in Whitten’s medium for 24, 48 and 72 hours at 37ºC, 5% of CO2 and 100% of humidity. The in vitro development ranged from 56.6% to 100% after 72 hours. Expanded blastocysts were transferred to pseudopregnant recipients on the third day of the estrous cycle. Viable fetuses rates for embryos frozen to -24 or -32ºC at 0.3ºC/minute in 10% glycerol + 10% sucrose, 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly, 10% glycerol + 0.1%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Embryo; Cryopreservation; Sucrose; Royal jelly.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400009

Cryopreservation of mouse morulae through different methods: slow-freezing, vitrification and quick-freezing
Provedor de dados: 62
Autores: MELLO,Marco Roberto Bourg de; QUEIROZ,Vinícius Seixas; LIMA,Alessandra Sobreira de; TAVARES,Liliam Mara Trevisam; ASSUMPÇÃO,Mayra Elena Ortiz D'Avila; WHEELER,Mathew B.; VISINTIN,José Antonio.
The in vitro and in vivo development of mouse morulae after cryopreservation through different methods was examined. The slow-freezing involved an equilibration in 1.5M ethylene glycol (EG) and cooled at 0.5; 0.7; 1.0 or 1.2ºC/minute. The vitrification involved a 3 minutes equilibration in 20% EG and 60 seconds in solution containing 40% EG, 18% ficoll and 10.26% sucrose. The quick-freezing involved an equilibration in 3M EG + 0.3M sucrose for 5 minutes and 2 minutes in nitrogen vapor. In all three methods the straws were thawed in air for 10 seconds and in water at 25ºC for 20 seconds and the embryos cultured in vitro for 72 hours to estimate blastocyst rate. To assess viability in vivo, frozen morulae as well as fresh embryos were transferred into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Embryo; Mouse.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000400003

Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions
Provedor de dados: 5
Autores: Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno.
A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P &lt; 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P &lt; 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied....
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf

Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss
Provedor de dados: 52
Autores: Ninhaus-Silveira,Alexandre; Foresti,Fausto; Tabata,Yara Aiko; Rigolino,Marcos Guilherme; Veríssimo-Silveira,Rosicleire.
Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oncorhynchus mykiss; Cryopreservation; Sex reversal; Gynogenesis; Semen.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009

Cryopreservation of sperm in marine fish
Provedor de dados: 5
Autores: Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R.
Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results....
Tipo: Text
Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf

Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source
Provedor de dados: 58
Autores: Arias,Cesar Andres Diaz; Marques,Daniela de Araujo Viana; Malpiedi,Luciana Pellegrini; Maranhão,Andrea Queiroz; Parra,Dulcineia Abdalla Saes; Converti,Attilio; Pessoa Junior,Adalberto.
Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment anti low density lipoprotein (-) under the control of the alcohol oxidase promoter have been investigated in this study. In particular, it was evaluated if, and eventually how, the carbon source (glucose or glycerol) used in the preculture preceding cryopreservation in 20% glycerol influences both cell and antibody fragment productions either in flasks or in bioreactor. Although in flasks the volumetric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pichia pastoris; ScFv antibody fragment; Cryopreservation; Expression; Carbon source.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300419

Date palm micropropagation: Advances and applications
Provedor de dados: 64
Autores: Al-Khayri,Jameel Mohammed; Naik,Poornananda Madhava.
ABSTRACT Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Somatic embryogenesis; Somaclonal variation; Organogenesis; Molecular marker.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000400347

Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability
Provedor de dados: 52
Autores: Ismail,Mohd Iswadi; Osman,Khairul; Ibrahim,Siti Fatimah; Jaafar,Farah Hanan Fatihah; Ghani,Nur Azianie Abd; Zainalabidin,Fazly Ann; Othman,Abas Mazni.
The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm²) and single tubing-type canister (4π x 90 mm²). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p&lt;0.05). Significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Sperm; Canister; Motility; Viability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200006

Efeito de diferentes soluções crioprotetoras na viabilidade da adipócitos bovinos após criopreservação (2009).
Provedor de dados: 14
Autores: SILVA, W. G.; SCHWARZ, D. G. G.; OLIVEIRA, C. T. S. A. M.; BRAVO, R. P. B.; SIQUEIRA, L. G.; FERRÃO, F. R.; MARTINS, C. F.
bitstream/item/78157/1/p2009-045.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Bovine.; Bovino; Germoplasma.; Beef cattle; Cryopreservation; Germplasm.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/747796

Effect of cryopreservation of sheep semen related to its viability and acrosomal status
Provedor de dados: 101
Autores: Hernández,P.J.E; Fernández,R.F; Rodríguez,S.J.L; Juárez,R.E; Soto,M.Y.G; García,R.A.D.
In the present research, the effects of cryopreservation on the viability and acrosomal status of sheep sperms were analyzed. Forty five ejaculates were obtained by the artificial vagina method for analysis, evaluating in fresh semen the following characteristics: volume (Vol), progressive motility (PM), viability percentage (Viab), normal morphology (NM), spermatic concentration (Concentr); as well as its viability and acrosomal status, The last two parameters were evaluated staining with fluorescein isothiocyanate conjugated with Arachis hypogaea lectin and propidium iodide (FITC-PNA/IP), in which there were different patterns of staining: live sperms without acrosomal reaction (VsRA), live with acrosomal reaction (VcRA), dead without acrosomal reaction...
Tipo: Journal article
Palavras-chave: Cryopreservation; Viability; Acrosomal reaction; FITC-PNA/IP; Sheep semen.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000200003

Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana
Provedor de dados: 69
Autores: Li,Zhongai; Du,Yaqiong; Wang,Zicheng.
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arabidopsis thaliana were successfully conserved in vitro by cryopreservation methods. Results: The effects of osmotic stress due to cryoprotectants during pretreatment and of storage at -196ºC on the stability, the efficiency of genetic transformation and the expression level of exogenous gene were analyzed in Arabidopsis. The results showed that there had not any significant increasing in the efficiency of genetic transformation after cryopreservation, and...
Tipo: Journal article
Palavras-chave: Arabidopsis thaliana; Cryopreservation; Real-time PCR; Transformation efficiency.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600012

Effect of Intraperitoneal Administration of Desialylated Erythropoietin on the Follicular Survival in Cryopreserved Canine Ovaries after Xenotransplantation
Provedor de dados: 19
Autores: Hirayama, Yukari; Inoue, Kou-suke; Suzuki, Hiroshi; 鈴木, 宏志.
The effect of intraperitoneal administration of desialylated erythropoietin (asialo EPO) on the follicular reserve of cryopreserved canine ovarian tissues was examined using non obese diabetic-severe combined immunodeficient (NOD-SCID) mice was examined. Vitrified-warmed canine ovarian tissues were placed under the dorsal skin of mice, and were then treated with saline or 400 or 4,000 U/kg/day of asialo EPO by intraperitoneal injection from one day before transplantation for 5 days. Transplanted tissues were removed and subjected to a histological examination 4 weeks after the transplantation. Without asialo EPO treatment, the mean number of primordial follicles in the cryopreserved ovarian tissues had decreased from 4.3/mm2 to 0.2/mm2 at 4 weeks after...
Palavras-chave: Canine; Cryopreservation; Ovary; Transplantation.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3856

Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
Provedor de dados: 48
Autores: MOZDARANI,HOSSEIN; MORADI,SHABNAM Z.
This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration...
Tipo: Journal article
Palavras-chave: Cryopreservation; Vitrification; Mouse embryo; Viability; Chromosome abnormalities.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004

Effects of cryoprotectant and plunging temperature in liquid nitrogen on the in vitro and in vivo development of murine morulae
Provedor de dados: 62
Autores: PANTANO,Thaís; MELLO,Marco Roberto Bourg de; GARCIA,José Fernando; HO,Linda Lee; VISINTIN,José Antonio.
The effects of plunging temperature in liquid nitrogen and cryoprotectant dilution methods were evaluated for compacted mouse morulae frozen in 1.5 M ethylene-glycol (E), 1.5M propylene-glycol (P) or 1.4M glycerol (G). Morulae were equilibrated for 10 minutes in cryoprotectant solution and loaded into 0.25 ml straws with cryoprotectant solution in 3 columns (groups E1, P1, G1) or cryoprotectant in the center and PBS in the lateral columns (E2, P2). Straws were cooled at 0.5ºC/min to -25 or -30ºC and plunged into liquid nitrogen. Straws were thawed in water at 22ºC for 20 seconds. Cryoprotectant was diluted in 3 steps for group G1 and in one step for groups E1 and P1 (direct transfer to PBS + 10% FCS) and E2 and P2 (shaken to mix the 3 columns before...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryos; Cryopreservation; Glycerol; Ethylene glycol; Propanediols.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300012

Effects of cryoprotectants and low temperatures on hatching and abnormal embryo development of Prochilodus lineatus (Characiformes: Prochilodontidae)
Provedor de dados: 83
Autores: Costa,Raphael S.; Souza,Fabrício M. S. de; Senhorini,José A.; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,Alexandre.
ABSTRACT This study evaluated the effect of the cryoprotectants and the low temperatures on the embryonic development of Prochilodus lineatus, describing their main morphological alterations. On chilling sensitivity test, the survival rates at the twenty somites stage (20S) were 53.6% at 0ºC, and 100% in 5ºC. To test toxicity, the embryos were exposed to a graded series of 1,2-Propanediol (PROP), dimethyl sulfoxide (Me2SO4) and glycerol (GLY), terminating in a solution of high osmolarity. There was no significant difference in the embryos survival of toxicity test between series of PROP and Me2SO4 in the 6S and 20S. In the cooling protocols, were evaluated the effects of low temperature associated with cryoprotectants. At 5ºC, PROP showed survival rates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cooling; Cryopreservation; Injuries; Morphology; Toxicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000300201

Effects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method
Provedor de dados: 56
Autores: Esteves,S.C.; Spaine,D.M.; Cedenho,A.P.
The objective of the present study was to investigate the effects of the direct addition of pentoxifylline (PF) to the ejaculates of men with poor sperm quality before freezing on post-thaw sperm motility, viability, acrosome integrity, and agonist-induced acrosome reaction. Semen specimens from 16 infertile men with impaired sperm count and motility (oligoasthenozoospermia) were divided into two equal aliquots: one received no treatment (control) while the other was incubated with 5 mM PF (treated). Both aliquots were cryopreserved by the liquid nitrogen vapor method. Motility was assessed according to WHO criteria. Acrosome integrity and spontaneous and calcium ionophore-induced acrosome reactions were assessed with fluorescein isothiocyanate-conjugated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrosome; Cryopreservation; Human spermatozoa; Ionophore; Pentoxifylline; Oligoasthenozoospermia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700014

Effects of preculture with sucrose and aba on cell suspensions water status and its relation with vitrification resistance
Provedor de dados: 93
Autores: SEIJO,GUILLERMO.
Changes in cell water relations during precultures were followed in an attempt to understand the mechanism of cell hardening for cryopreservation by vitrification. Medium containing 0.4 M sucrose (psiw=-1.45 MPa) and containing 5 mg L-1 of ABA (MT psiw=-0.73 MPa and MS psiw=-0.48 MPa) were used to harden cell suspensions of orange and carrot. Preculture in these medium did not cause a significant decrease of cell viability, however, it improved the cell survival to PVS2 and liquid nitrogen expositions. When cells were inoculated into medium containing 0.4 M sucrose, the cell psiw decreased rapidly until reach the medium psiw , and turgor was also severely reduced or disappeared. Subsequently, cell psis began to decrease and after 48 h of treatment in low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preculture; Cell suspension; Sucrose; ABA; Cryopreservation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312000000200007

Essai de cryoconservation des gamétophytes de l'algue alimentaire Undaria pinnatifida (Laminariale)
Provedor de dados: 5
Autores: Arbault, Suzanne; Renard, Philippe; Pérez, Rene; Kaas, Raymond.
Low temperature tolerance was determined for gametophytes of food alga Undaria pinnatifida according to various cryopreservation methods. Survival durations after storage in a medium containing 28% glycerol at −80 °C or in a 5-10% glycerol solution at −196 °C were only 9 and 4 days respectively. On the other hand, cooling at 5 °C/min. and storage at −15 °C for 15 minutes, without cryoprotective medium, did not reduce viability but this was highly decreased by storage for 15 minutes at −30 or −196 °C, with or without DMSO. The morphological cooling injuries were depigmentation and/or a contraction of the cells.
Tipo: Text
Palavras-chave: Algue; Cryoconservation; Gamétophyte; Undaria pinnatifida; Alga; Cryopreservation; Gametophyte; Undaria pinnatifida.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00189/30071/28559.pdf

Establishment of a cryopreserved biobank for the Culture Collection of Freshwater Microalgae (CCMA-UFSCar), São Paulo, Brazil
Provedor de dados: 49
Autores: Tessarolli,Leticia Piton; Day,John Godfrey; Vieira,Armando Augusto Henriques.
Abstract The Culture Collection of Freshwater Microalgae (CCMA-UFSCar, Coleção de Culturas de Microalgas de Água-Doce), based at the Universidade Federal de São Carlos, plays an important role in underpinning Brazilian microalgal research, providing biological materials, substrates and training personnel for a large proportion of the past and current projects in this area. However, recent efforts to expand the culture catalogue/holdings have reached a ceiling because of the logistical practicality of maintaining actively growing cultures. In order to reduce the costs associated with the maintenance regime of cultures, efforts were initiated on the establishment of a cryopreserved biobank for long term maintenance of cultures, thus minimizing the efforts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Freshwater microalgae; Biobank.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032017000200201

First study in cryopreserved Crassostrea angulata sperm
Provedor de dados: 5
Autores: Riesco, Marta F.; Felix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa.
Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a...
Tipo: Text
Palavras-chave: Crassostrea angulata; Sperm; Cryopreservation; Motility; Viability; Fertilization.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00333/44432/44098.pdf

Follicular Loss of the Cryopreserved Canine Ovary after Xenotransplantation
Provedor de dados: 19
Autores: Ishijima, Takako; Abe, Yasuyuki; Suzuki, Hiroshi; 鈴木, 宏志.
The effect of cryopreservation and subsequent xenotransplantation on the follicular reserve of the canine ovary by using non obese diabetic-severe combined immunodeficient (NOD-SCID) mice was examined. Vitrified-warmed canine ovarian tissues were placed into the ovarian bursa of mice, and then were removed and subjected to histological examination at 4 weeks after the transplantation. Over 30% of primordial follicles and 65% of early primary follicles survived after cryopreservation. However, regardless of breed or age, percentages of survived primordial follicles and early primary follicles after the transplantation ranged from 0-7% and from 0-15%, respectively. These results indicate that the majority of primordial follicles and early primary follicles...
Palavras-chave: Canine; Cryopreservation; Ovary; Transplantation.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2802

"Freezebass" Setting up of a sperm cryobank for sea bass. Q5CT-2002-71209. Final report (01 January 2003 – 30 June 2005)
Provedor de dados: 5
Autores: Chatain, Beatrice; Poline, Olivier.
Objectives : Presently, most of marine fish farmers around the Mediterranean basin grow genetically wild fish strains, which have not been genetically improved for culture performance (growth, appearance, disease resistance etc.). It is inevitable that the present gap for genetically improved strains of marine fish, and their reliable supply, will be filled. It will ultimately dominate the competitive component of marine culture industries once they evolve into commodity-style agribusiness (with fish prices being set at the lowest possible cost of production and the best quality). Those countries, which can competitively develop and supply the improved strains, will enjoy the benefit (perhaps survival) for their local industries. They will avoid...
Tipo: Text
Palavras-chave: Pathogen free; Genetic origin; Sperm; Cryopreservation; Sea bass.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/rapport-6494.pdf

Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract
Provedor de dados: 83
Autores: Viveiros,Ana T.M.; Gonçalves,Antonio C.S.; Nascimento,Ariane F.; Leal,Marcelo C.
The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and post-thaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CASA; Characiformes; Cryopreservation; Ovaprim™; Sperm motility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100157

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions
Provedor de dados: 83
Autores: Garcia,Raycon Roberto Freitas; Vasconcelos,Ana Carina Nogueira; Povh,Jayme Aparecido; Oberst,Eneder Rosana; Varela Jr.,Antonio Sérgio; Corcini,Carine Dahl; Streit Jr.,Danilo Pedro.
Cryoprotectant solutions are used to protect the sperm from alterations caused by the low temperature in the cryopreservation process. We evaluated the quality of Colossoma macropomum semen after freezing, using dimethyl sulfoxide (DMSO) as a cryoprotectant, combined with two extender solutions (T1 - Solution 1: Glucose 90.0 g/L, Sodium Citrate 6.0 g/L, EDTA 1.5 g/L, Sodium Bicarbonate 1.5 g/L, Potassium Chloride 0.8 g/L, Gentamycin Sulphate 0.2 g/L, and T2 - Solution 2: Glucose 90.0 g/L, ACP(r)-104 10.0 g/L). Motility rate and motility time did not differ between T1 and T2 and were lower than fresh semen. The number of normal sperm was significantly different in treatments T1 (15.1%) and T2 (21.9%), and both showed a reduction in the percentage of normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Cryoprotectant solution; Mitochondrial functionality; Motility; Spermatic DNA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300599

Germinability after desiccation, storage and cryopreservation of seeds from endemic Encholirium Mart. ex Schult. &amp;amp; Schult. f. and Dyckia Schult. &amp;amp; Schult. f. species (Bromeliaceae)
Provedor de dados: 39
Autores: Tarré,Erika; Pires,Bárbara Balzana Mendes; Guimarães,Ana Paula Mançano; Carneiro,Leonardo Alves; Forzza,Rafaela Campostrini; Mansur,Elisabeth.
(Germinability after desiccation, storage and cryopreservation of seeds from endemic Encholirium Mart. ex Schult. &amp;amp; Schult. f. and Dyckia Schult. &amp;amp; Schult. f. species (Bromeliaceae)). Seed storage procedures require previous determination of optimal temperature and light conditions for germination, as well as of tolerance to desiccation and low temperatures. The aim of this paper was to study the effects of desiccation, storage at low temperatures and cryopreservation on the germinability of seeds of six Encholirium and two Dyckia species, which were selected according to vulnerability criteria. Initial germinability of newly harvested seeds varied from 35 to 95%. Seeds presented photoblastic behaviour since light was necessary to induce or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Espinhaço mountain range; Germination; Orthodox seeds; Seed conservation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062007000400003

Germinação de sementes de Ipomoea cynanchifolia após armazenamento em diferentes temperaturas e escarificação.
Provedor de dados: 14
Autores: SANTOS, I. R. I.; SALOMAO, A. N.
Germination of Ipomoea cynanchifolia seeds after storage at different temperatures and scarification. Abstract ? Ipomoea cynanchifolia Meisn., Convolvulaceae, is a wild relative of Ipomoea batatas (L.) Lam. (sweet potato). In this study, seeds with 7.8% water content were stored in a germinator (25 °C), a freezer (-18 °C) and in liquid nitrogen (-196 °C) for seven days. After storage, seeds underwent a decontamination treatment for 15 min, followed by three rinses with sterile distilled water, and after that seeds were scarified, by means of a cut in the opposite pole to the hilum made with nail pliers. Non-scarified (control, ESC-) and scarified (ESC +) seeds were sown in Petri dishes between filter paper discs soaked with water equivalent to three times...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Escarificação; Criopreservação; Dormência; Cryopreservation.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1116845

Germination and gametophyte development of Cyathea corcovadensis (Raddi) Domin (Cyatheaceae) from spores stored at low temperatures
Provedor de dados: 3
Autores: Marcon, Catiuscia; Silveira, Tatieli; Droste, Annette.
The tree fern Cyathea corcovadensis (Raddi) Domin is an endangered species in the state of Rio Grande do Sul, Brazil. It currently occurs only in the northern segment of the coastal region. Spore storage would help in conservation programs since it maintains genetic variability and provides material for in vitro cultures. Current study evaluates the effect of low temperatures combined to different spore storage times on the germination and initial gametophyte development of C. corcovadensis. Spores were divided into two groups: spores of the first group were sowed immediately in Meyer culture medium with nystatin, at pH 4.0, while spores of the second group were stored at 7, -20 and -196°C during 60, 120, 180, and 365 days and then sowed in the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.00.00.00-6 conservation; In vitro culture; Tree fern; Cryopreservation; Abiotic factor Ciências Biológicas.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/24318

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera
Provedor de dados: 5
Autores: Hui, Belinda; Vonau, Vincent; Moriceau, Jacques; Tetumu, Roger; Vanaa, Vincent; Demoy-schneider, Marina; Suquet, Marc; Le Moullac, Gilles.
Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry.  Spermatozoa were cryopreserved with CPA 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23 % lower than that of fresh sperm. When using thawed...
Tipo: Text
Palavras-chave: Cryopreservation; Spermatozoa; Fertility; Larval rearing; Pearl oyster; Pinctada margaritifera.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15098/12446.pdf

In vitro evaluation of canine spermatozoa cryopreserved in different extenders
Provedor de dados: 45
Autores: Oliveira,E.C.S.; Juliani,G.C.; Marques Jr.,A.P.; Henry,M.
The efficacy of three extenders, tris-egg yolk-5% ethylene glycol (T1), lactose-egg yolk-5% ethylene glycol (T2) and lactose-egg yolk-5% dimethyl formamide (T3) on preserving the viability of post-thawing canine spermatozoa was evaluated. Three ejaculates per dog were obtained of five animals. The semen was packaged in 0.5ml straws and cooled to 4°C for 120min. The straws were frozen 4cm above the nitrogen level for 15min and thawed in water-bath at 37°C for 60sec and at 75°C for 7sec. Progressive motility and vigour were evaluated immediately after thawing (time 0) and at 30, 60, 90 and 120min. Structural and functional integrity of plasma membrane of the spermatozoa were evaluated, respectively, by fluorescent staining probes and hypoosmotic swelling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Semen; Cryopreservation; Ethylene glycol; Dimethyl formamide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600021

In Vitro Excystation and Cryoreservation of Ovine and Caprine Sarcocystis species
Provedor de dados: 19
Autores: Horn, Karin; Ono, Kenichiro; Heydorn, Alfred Otto.
This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39OC in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additional sonication of pretreated sporocysts increased excystation rates as compared to not sonicated controls. Excystation rates for 1-9 months old sporocysts were 77% for S.capracanis, 77% for S.hircicanis, 72% for S.arieticanis and 92% for S.ovicanis. Sporozoite suspension as...
Palavras-chave: Cryopreservation; In vitro excystation; Sarcocystis spp.; Sporozoites.
Ano: 1991
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/154

Incidence of apoptotic cells after vitrification in canine ovarian tissues
Provedor de dados: 19
Autores: Hariya, Madoka; Suzuki, Hiroshi.
Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported, the degree of cryoinjury after the vitrification has not yet been fully examined. Since cryoinjury after vitrification of ovaries has been reported in bovine and humans, this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL assay. Ovaries were collected from immature bitches. The ovarian tissue cubes were pretreated with 1 M DMSO. Subsequently, DAP 213 solution (2 M DMSO, 1 M acetamide, 3 M propylene glycol) was added. The cryotubes containing the ovarian tissues were placed on ice for 5 min, then plunged directly into liquid nitrogen. After warming with 0.25 M sucrose, cryopreserved...
Palavras-chave: Cryopreservation; Dog; Follicle; Ovary.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4535

Isolamento, cultivo criopreservação de células somáticas de mamíferos silvestres para formação de um banco de germoplasma.
Provedor de dados: 14
Autores: MARTINS, C. F.; CUMPA, H. C. B.; CUNHA, E. R. da; SILVA, C. G. da; GAMA FILHO, J. B. da; BORGES, L. A.
bitstream/item/100353/1/comtec-175.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Animal silvestre; Animal conservation; Genebank.; Criopreservação; Germoplasma; Mamífero.; Biotechnology; Cryopreservation; Reproduction; Wild animals.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/981278

Karyotype of cryopreserved bone marrow cells
Provedor de dados: 56
Autores: Chauffaille,M.L.L.F.; Pinheiro,R.F.; Stefano,J.T.; Kerbauy,J.
The analysis of chromosomal abnormalities is important for the study of hematological neoplastic disorders since it facilitates classification of the disease. The ability to perform chromosome analysis of cryopreserved malignant marrow or peripheral blast cells is important for retrospective studies. In the present study, we compared the karyotype of fresh bone marrow cells (20 metaphases) to that of cells stored with a simplified cryopreservation method, evaluated the effect of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) as an in vitro mitotic index stimulator, and compared the cell viability and chromosome morphology of fresh and cryopreserved cells whenever possible (sufficient metaphases for analysis). Twenty-five bone marrow...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cryopreservation; Karyotype; Bone marrow cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700004

Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa
Provedor de dados: 5
Autores: Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland.
The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use...
Tipo: Text
Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf

Lyophilized allografts without pre-treatment with glutaraldehyde are more suitable than cryopreserved allografts for pulmonary artery reconstruction
Provedor de dados: 56
Autores: Olmos-Zúãiga,J.R.; Jasso-Victoria,R.; Díaz-Martínez,N.E.; Gaxiola-Gaxiola,M.O.; Sotres-Vega,A.; Heras-Romero,Y.; Baltazares-Lipp,M.; Baltazares-Lipp,M.E.; Santillán-Doherty,P.; Hernández-Jiménez,C.
Various methods are available for preservation of vascular grafts for pulmonary artery (PA) replacement. Lyophilization and cryopreservation reduce antigenicity and prevent thrombosis and calcification in vascular grafts, so both methods can be used to obtain vascular bioprostheses. We evaluated the hemodynamic, gasometric, imaging, and macroscopic and microscopic findings produced by PA reconstruction with lyophilized (LyoPA) grafts and cryopreserved (CryoPA) grafts in dogs. Eighteen healthy crossbred adult dogs of both sexes weighing between 18 and 20 kg were used and divided into three groups of six: group I, PA section and reanastomosis; group II, PA resection and reconstruction with LyoPA allograft; group III, PA resection and reconstruction with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary artery graft; Cryopreservation; Lyophilization; Blood vessel bioprosthesis; Prosthesis design; Vascular procedures.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200701

Optimizing sperm cryopreservation in great scallop (Pecten maximus)
Provedor de dados: 5
Autores: Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian.
A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher...
Tipo: Text
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf

Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed
Provedor de dados: 45
Autores: Sprícigo,J.F.W.; Leme,L.O.; Guimarães,A.L.; Oliveira Neto,J.C.; Silva,P.C.P.; Moreira,N.H.; Pivato,I.; Silva,B.D.M.; Ramos,A.F.; Dode,M.A.N.
ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P&lt; 0.05). Among the most representative PL was the phosphatidylcholine [PC (32:0) + H]+; [PC (34:1) + H]+ and [PC (36:4) + H]+. Beyond the PL, MALDI revealed some triglycerides (TG), including PPL (50:2) + Na+, PPO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; Embryo; Cryopreservation; Lipids; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300837

Physalis peruviana seed storage
Provedor de dados: 91
Autores: Souza,Cíntia L. M. de; Souza,Manuela O. de; Oliveira,Ronaldo S.; Pelacani,Claudineia R.
ABSTRACT Physalis peruviana belongs to Solanaceae family and has a high nutritional and nutraceutical potential. The production is intended for fruit consumption and the propagation is mainly by seeds. This study aimed to evaluate the influence of priming on the kinetics of germination of P. peruviana seeds stored at different temperatures. The seeds were stored at 5 and 25 °C in a chamber saturated with zinc chloride solution and in liquid nitrogen (-196 °C). Every 4 months, the seeds were removed from storage for evaluation of germination and moisture content in the laboratory and emergence and development of seedlings in greenhouse. During the last evaluation at 16 months, the seeds under the same conditions were subjected to salt stress. The moisture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Priming; Cryopreservation; Salt solution; Viability.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000300263

Post-thaw acrosomal viability and reaction in sperm obtained from equine epididymis tail
Provedor de dados: 101
Autores: Hernández,P.J.E; Fernández,R.F; Rodríguez,S.J.L; Soto,M.Y.G; Verona,J.E.H; García,R.A.D.
In order to evaluate the post-thaw effect on acrosomal viability and reaction of sperm collected from equine epididymis tail, 62 testes were collected and transported at 4°C. Epididymis was dissected to perform the retrograde flushing of the epididymis tail , using 10 mL of a diluent based on skim milk (INRA 82). The diluent obtained with the spermatic content was recovered in graduated collecting tubes and 1mL of each sample was taken to be evaluated. For evaluations, the samples having a minimum of 30% of progressive motility were selected. The evaluations were on: percentage of live sperm, normal morphology, sperm concentration, percentage of live sperm without acrosomal reaction (VsRA) and live with acrosomal reaction (VcRA), evaluating this indicator...
Tipo: Journal article
Palavras-chave: Cryopreservation; Viability; Acrosomal reaction; FITC-PNA/IP; Sperm; Epididymis; Equine.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000200004

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa
Provedor de dados: 5
Autores: Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky.
High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J...
Tipo: Text
Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf

Recuperação e avaliação de espermatozóides obtidos de epidídimos bovinos resfriados por diferentes períodos (2009).
Provedor de dados: 14
Autores: FERRÃO, F. R.; SILVA, F. B. da; SCHWARZ, D. G. G.; OLIVEIRA, C. T. S. A. M. DE; SIQUEIRA, L. G. B.; BLUME, G. R.; COSTA, P. M.; MARTINS, C. F.
bitstream/item/78289/1/p2009-086.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Bovinae; Sperm; Gene bank.; Bovino; Banco de Germoplasma; Criopreservação; Espermatozóide; Reprodução Animal.; Animal breeding; Cattle; Cryopreservation; Germplasm.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/737112

Recuperação e criopreservação de espermatozóides do epidídimo de bovinos mortos para uso na inseminação artificial ou fecundação in vitro.
Provedor de dados: 14
Autores: MARTINS, C. F.; DODE, M. N.; SERENO, J. R. B.; COSTA, P. de M.; SAUERESSIG, M. G.; RUMPF, R.
bitstream/item/95713/1/comtec-151.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Bovinae; Sperm; Gene bank.; Bovino; Banco de Germoplasma; Criopreservação; Espermatozóide; Reprodução Animal.; Animal breeding; Germplasm.; Cryopreservation; Cattle.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570682

Reproductive bio-technology for the guide dog for the blind
Provedor de dados: 19
Autores: 鈴木, 宏志.
Although guide dogs make a remarkable contribution to the quality of life of the blind people, many countries suffer from an acute shortage of guide dogs such that not all those who need them are able to be supplied. One of the important challenges from the point of view of animal and veterinary science as well as social welfare is to establish a stable and effective breeding and reproduction system for guide dogs for the blind. Although the development and extension of assisted reproductive techniques such as artificial insemination, embryo transfer and in vitro fertilization has been remarkable, in canines, it is not a successful as other mammalian spices such as mice, pigs, cows and humans. In this review, recent advances of cryopreservation of sperm,...
Palavras-chave: Guide dog; Artifical insemination; Embryo transfer; Recipient; Cryopreservation; イヌ; 人工授精; 移植; 受容雌; 凍結保存.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4511

Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)
Provedor de dados: 52
Autores: Ninhaus-Silveira,Alexandre; Foresti,Fausto; Veríssimo-Silveira,Rosicleire; Senhorini,José Augusto.
Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semen characteristics; Cryopreservation; Brycon cephalus; Ultrastructure; Fish.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500015

Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae
Provedor de dados: 5
Autores: Suquet, Marc; Le Mercier, Alain; Rimond, Flore; Mingant, Christian; Haffray, Pierrick; Labbe, C.
Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, to estimate Pacific oyster larval quality after thawing, and 2) select complementary tools allowing an early and reliable estimation of their quality. Oyster larvae were reared for 25 h after fertilization at 19 degrees C and cryopreserved at early D-stage. Then, thawed larvae were incubated in 2-L beakers. At 2 days post fertilization, the survival rate of thawed Pacific oyster larvae was lower than that of fresh larvae for only one experiment (Experiment 3) among the four identical...
Tipo: Text
Palavras-chave: ASMA; CASA; Crassostrea gigas; Cryopreservation; D-larvae.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19934/17761.pdf

Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm
Provedor de dados: 5
Autores: Chereguini, Olvido; Cal, Rosa Maria; Dreanno, Catherine; Ogier De Baulny, Bénédicte; Suquet, Marc; Maisse, Gérard.
Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 degrees C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.
Tipo: Text
Palavras-chave: Turbot; Sperm; Storage; Cryopreservation.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00176/28724/27173.pdf

Sperm motility in European hake, Merluccius merluccius, and characterization of its spermatozoa concentration and volume, spermatocrit, osmolality and pH
Provedor de dados: 5
Autores: Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Mayer, Ian; Severe, Armelle; Bouquet, Jean-marie; Geffen, Audrey J.; Utne-palm, Anne Christine; Kjesbu, Olav Sigurd.
Due to stock declines there is a growing interest in farming of European hake Merluccius merluccius. Thus, knowledge of its sperm biology is of importance not only for purposes of broodstock management, but also for the development of sperm preservation techniques. Hake sperm were collected from mature males caught during the summer-early autumn waters off western Norway and during the winter-early spring in the Bay of Biscay (France). Sperm quality characteristics were assessed after storage at 4 degrees C for 25 +/- 14 h. Average (+/- SD) values for Norwegian and French samples respectively were (i) sperm volume: 3.9 +/- 4.0 and 2.6 +/- 4.0 ml; (ii) spermatozoa concentration: 6.6 +/- 3.2 and 13.9 +/- 5.1 x 10(9) spermatozoa/ml; (iii) spermatocrit: 80.2...
Tipo: Text
Palavras-chave: Cryopreservation; Merluccius merluccius; Osmolality; PH; Sperm motility; Spermatocrit.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11393/8021.pdf

Strategies for commercialization of cryopreserved fish semen
Provedor de dados: 96
Autores: Tiersch,Terrence R.
Initial success in sperm cryopreservation occurred at about the same time for aquatic species and livestock. However, in the 50 plus years since then cryopreserved sperm of livestock has grown into a billion-dollar global industry, while cryopreserved sperm of aquatic species remains a research activity with little commercial application despite work in more than 90 species and more than 200 published reports. Most research work has focused on large-bodied culture and sport fishes, such as salmon, trout, carp, and catfish, and mollusks such as commercially important oyster and abalone species. However, only a few studies have addressed sperm cryopreservation in small fishes such as zebrafish, or in endangered species. Overall, this work has yielded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic species; Cryopreservation; Germplasm; Repositories.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300003

The importance of glucose for the freezing tolerance/intolerance of the anuran amphibians Rana catesbeiana and Bufo paracnemis
Provedor de dados: 57
Autores: STEINER,A. A.; PETENUSCI,S. O.; BRENTEGANI,L. G.; BRANCO,L. G. S.
Several species of terrestrially hibernating frogs, turtles and insects have developed mechanisms, such as increased plasma glucose, anti-freeze proteins and antioxidant enzymes that resist to freezing, for survival at subzero temperatures. In the present study, we assessed the importance of glucose to cryoresistance of two anuran amphibians: the frog Rana catesbeiana and the toad Bufo paracnemis. Both animals were exposed to -2ºC for measurements of plasma glucose levels, liver and muscle glycogen content, haematocrit and red blood cell volume. Frogs survived cold exposure but toads did not. Blood glucose concentration increased from 40.35 ± 7.25 to 131.87 ± 20.72 mg/dl (P &lt; 0.01) when the frogs were transferred from 20 to -2ºC. Glucose accumulation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bufo paracnemis; Rana catesbeiana; Freeze tolerance; Cryopreservation; Glucose.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200017

The quality of great scallop (Pecten maximus) sperm after thawing
Provedor de dados: 5
Autores: Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian.
Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol.  Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm...
Tipo: Text
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf

The use of reduced glutathione (GSH) as antioxidant for cryopreserved sperm in dogs
Provedor de dados: 45
Autores: Angrimani,D.S.R.; Nichi,M.; Brito,M.M.; Kawai,G.K.V.; Rui,B.R.; Losano,J.D.A.; Vieira,N.M.G.; Francischini,M.C.P.; Cruz,D.S.G.; Queiroz-Hazarbassanov,N.; Massoco,C.O.; Vannucchi,C.I.
ABSTRACT The aim of this study was to evaluate the effect of supplementation with different concentrations of reduced glutathione GSH (0; 5; 7.5; 10mM) in the extender for cryopreservation in dogs with evaluations performed after glycerolization (chilled) and thawing (thawed). For this purpose, we used 8 dogs and two semen collections were performed in a weekly interval, totaling 16 semen samples. The sperm were analyzed by automatic sperm motility (CASA) and flow cytometry analysis of mitochondrial potential (JC1 dye) and membrane/acrosome integrity (FITC-PI dyes). We evaluated subjectively the membrane and acrosome integrity, mitochondrial activity and DNA integrity. Seminal plasma was evaluated for lipid peroxidation (TBARS concentration). Chilled and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reactive oxygen species; Cryopreservation; Antioxidant; Canine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200419

Tropical seed species' responses to liquid nitrogen exposure
Provedor de dados: 61
Autores: Salomão,Antonieta Nassif.
The ability to tolerate ultra-low temperature (-196 ºC) exposure was evaluated in 66 tropical orthodox seed species of 21 botanical families from the Cerrado (Brazilian Savannah) and Atlantic Forest Brazilian biomes. Liquid nitrogen had no effect on the germinability of 51 seed species. The stimulatory effect of cryogenic temperature on germinability, with or without subsequent chemical scarification, was observed in nine seed species with deep physical dormancy, or heterogeneous levels of seed hardness, or with no dormancy. Significant reduction in germinability occurred in six seed species, presumably because of factors acting individually or in combination on these seeds, such as inappropriate moisture content, or the potentially detrimental effect of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Ex situ conservation; Seed dormancy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200008

Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
Provedor de dados: 42
Autores: DEMARCHI,GRASIELA; STEFENON,VALDIR M.; STEINER,NEUSA; VIEIRA,FELIPE N.; VESCO,LIRIO L. DAL; GUERRA,MIGUEL P.
This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Ex situ conservation; In vitro culture; Plant germplasm conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057

Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)
Provedor de dados: 83
Autores: Gárriz,Ángela; Miranda,Leandro A.
In the present study it was showed for the first time the ultrastructural morphology of O. bonariensis sperm using electron microscopy techniques. Different kinds of abnormalities were described in fresh and post thawed sperm caused by crogenic protocols. Pejerrey spermatozoon is uniflagellated and is differentiated into three parts: a small roundish head (~1.80µm in length and 1.67µm in width), a midpiece or transitional region (~1.11µm in length and 1.56µm in width), and a long tail or flagellum (~29.08µm). Samples of fresh and post thawed sperm showed evidence of morphological anomalies affecting various intracellular compartments. Spermatozoa with swollen, ruptured, or absent membranes in the head showing excess of cytoplasm, and with alteration of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Morphology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000400831

Use of Inert Gases for the Preservation of Nuclear Blood Cells
Provedor de dados: 52
Autores: Khudyakov,Andrey Nikolaevich; Polezhaeva,Tatyana Vitalyevna; Zaitseva,Oksana Olegovna; Sergushkina,Marta Igorevna; Solomina,Olga Nurzadinovna.
Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clathrates; Cryopreservation; Xenon; Krypton; Leukocytes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100404

Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes
Provedor de dados: 65
Autores: Santos,Rodrigo Marques dos; Barreta,Marcos Henrique; Frajblat,Marcel; Cucco,Diego Córdova; Mezzalira,Joana Claudia; Bunn,Silvério; Cruz,Fabiano Buss; Vieira,Arnaldo Diniz; Mezzalira,Alceu.
The objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitrification; Cryopreservation; Oocytes; Bovine; Vacuum.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024

Viability of dourado embryos cooled in different cryoprotectant solutions
Provedor de dados: 86
Autores: Fornari,Darci Carlos; Streit Junior,Danilo Pedro; Galo,Juliana Minardi; Guerreiro,Luís Jayme; Povh,Jayme Aparecido; Barrero,Nelson Mauricio Lopera; Oliveira,Diego de; Ribeiro,Ricardo Pereira.
The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (-8ºC for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Salminus brasiliensis; Cryopreservation; Fish embryo; Embryo preservation; Larvae eclosion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800060